منابع مشابه
Modulation of human prothrombin activation on phospholipid vesicles and platelets using monoclonal antibodies to prothrombin fragment 2.
Prothrombin contains two kringle domains that are removed during activation to the blood clotting enzyme alpha-thrombin. By analogy with other kringle-containing proteins the prothrombin kringles may play a role in the protein-protein interactions necessary for prothrombin activation. Four monoclonal antibodies to prothrombin kringle 2 have been produced against human prothrombin, and a fifth m...
متن کاملHuman prothrombin activation.
Human prothrombin has been purified from American Red Cross Factor IX concentrates. Studies of the activation of the human prothrombin with the use of sodium dodecyl sulfate electrophoretic analysis of activation products indicated that human prothrombin activation is similar to bovine prothrombin activation. Molecular weight analysis of human prothrombin and intermediated by sodium dodecyl sul...
متن کاملStructural transitions during prothrombin activation: On the importance of fragment 2
Prothrombin is activated to thrombin by the prothrombinase complex through sequential cleavage at two distinct sites. This occurs at sites of vascular injury in a highly regulated cascade of serine protease and cofactor activation, where activated platelets provide a suitable surface for protease/cofactor/substrate assembly. The precise structural and conformational changes undergone during the...
متن کاملMonoclonal antibodies specific for prothrombin fragment 1.2 and their use in a quantitative enzyme-linked immunosorbent assay.
Prothrombin fragment 1.2 (F1.2) is an activation peptide generated during a critical event of blood coagulation, the conversion of prothrombin to thrombin. As a marker of thrombin generation, F1.2 has clinical potential in assessing thrombotic risk and monitoring anticoagulant therapy. In developing a highly specific, monoclonal antibody-based immunoassay of human plasma F1.2, we generated six ...
متن کاملLeft atrial thrombin generation and prothrombin fragment 1+2.
Peverill and colleagues [1] recently reported differences in levels of prothrombin fragment 12 (F12) in blood obtained from the left atrium, the femoral artery and the femoral vein from 36 patients with mitral stenosis. They aimed (i) to compare arterial and venous levels of F12 in patients with normal clotting times, (ii) to examine and compare the relationship between arterial and venous l...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1993
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/39.4.559